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MARN365/265
Molecular Approaches
to Biological Oceanography
Instructors: Senjie Lin
3 credits, one 2-hr lectures per week (Wed,
2:00-4:00 pm), one 3hr lab per week (Fri,
9:30 am-12:30 pm). Offered at Avery Point
In general, this course will introduce all
major molecular biological techniques that
are useful for ecological studies. Lectures
will cover theories and principles behind
the techniques and highlight some technical
difficulties often encountered. Labs will
offer hands-on experience for students.
Typically, students will work in groups
of 2 to 3 on biological samples students
choose.
• Lecture 1: Introduction: Why molecular
techniques?
• Lab 1: Preparation of reagents and
samples
Lecture 2: Nucleic acid properties and DNA
isolation
• Lab 2: DNA isolation
• Lecture 3: PCR*
• Lab 3: PCR
• Lecture 4: DNA fingerprinting (RFLP,
AFLP, VNTR, etc)
• Lab 4: DNA restriction digestion
and gel electrophoresis.
• Lecture 5: Molecular cloning and
sequencing
• Lab 5: Gene cloning
• Lecture 6: Basic informatics
• Lab 6: Gene cloning (continued)
• Lecture 7: Molecular phylogeny
• Lab 7: Isolation of plasmids
• Lecture 8: Population genetics
• Lab 8: BLAST, alignment, and phylogeny
• Lecture 9: Gene transcription and
mRNA detection
• Lab 9: Isolation of RNA and synthesis
of cDNA
• Lecture 10: Translation and protein
detection
• Lab 10: RT-PCR and real-time qPCR
• Lecture 11: Genomics and proteomics
• Lab 11: RT-PCR and real-time qPCR
(continued)
• Lecture 12: Metagenomics
• Lab 12: Immunofluorescence
• Lecture 13: Application to Oceanography
• Lab 13: Work on project
• Lecture 14: Application to other
marine organisms
• Lecture 15: Study for final and work
on project
• Lab 14: Work on project
* PREMIER Biosoft International Developers
of Intuitive Software for the Molecular Biologist
kindly provides Beacon Designer for primer
and probe design in this course. To learn
more about this software, please check out
their website http://www.premierbiosoft.com/molecular_beacons/index.html.
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